It’s essential to have an internal standard for every metabolite measured for proper and reproducible metabolic profiling. IROA quantitation kits and IROA internal standard kits are tools that you can use for metabolic profiling. With IROA quantitation kits, you can make your own internal standards that mirror the metabolome to be measured. These kits contain all of the reagents that are required for labeling, quantitating, and identifying biochemical compounds in cell populations successfully.
With IROA 100, you can create yeast internal standards, identify and quantitate yeast metabolites. With IROA 200, you can create bacterial internal standards, identify and quantitate bacterial metabolites. And with IROA 300, you can create mammalian internal standards, identify and quantitate mammalian metabolites. Mammalian metabolic profiling kits include 13C yeast extract metabolite standard.
On the other Hand, IROA internal standard kits contain complex internal standards that are pre-labeled. It can be used for the identification of 100’s of metabolites across time and instrument. You will get ClusterFinder software and statistical analysis tools with any kit purchase.
Below, we are going to talk about the IROA metabolic profiling tools that contain 5% and 95% uniformly (U) 13C-labeled yeast extract. Consider going through the following sections of this article properly to know more in this regard.
IROA Mammalian Profiling Tools
IROA has introduced two quantitation kits for accurate mammalian metabolic profiling. This section covers some important things you need to know about these kits. Look through the below portion properly to learn about them.
1. IROA® Fluxomic Quantitation Kit
The kit has two components: Labeling Medium (liquid) and Component Mix (dry). The labeling medium includes 1 bottle of 250 mL EBSS/RPMI 1640 vitamin solution (1X) containing 1mg/mL 5% U-13C labeled glucose and 1 empty sterile bottle. You can store media following filter sterilization in it. The component mix includes 657.5mg 5% U-13C labeled yeast extract and amino acids.
This kit provides the reagents to fully label mammalian cells at 5% U-13C. You may use this kit to determine the flux of a specific precusor through metabolic pathways. The use of 5% helps identify all metabolic pools. The mechanism also helps eliminate non-biological signals in a completely non-targeted and unbiased manner.
2. IROA® Phenotypic Quantitation Kit
This IROA kit for mammalian metabolic profiling also contains labeling medium and component mix. The labeling medium includes 1 bottle of 250 mL EBSS/RPMI 1640 vitamin solution (1X) containing 1mg/mL 95% U-13C labeled glucose and 1 empty sterile bottle. The component mix includes 657.5mg 95% U-13C labeled yeast extract and amino acids.
This kit is designed for situations where it is not possible to label experimental samples in cell culture systems such as biopsies, field-grown plants, or large-scale fermentation.
Know about IROA Kits in Detail
To find more information about IROA metabolic profiling tools and 13C yeast extract metabolic standard, you can contact ‘IROA Technologies LLC’. You will find detailed information about the tools on their website. Thus, log on to iroatech.com. Also, browse through other blogs and articles to learn more about advanced metabolic profiling tools.